Last time, I went over my plan to extract DNA from 4 random samples from SE AK Tanner crab, collected in 2005. This year was chosen because it’s the earliest samples we have - therefore, if they’ve got good DNA, all should have good DNA.

Plan was to pellet the hemolymph and then treat as a tissue sample. Samples were randomly chosen and numbered 1-4. A Qiagen DNEasy Blood + Tissue kit was used, followed by running them on the Qubit.

Important notes on use of Qiagen kit:

  • Incubated for ~40 min with Protease K (until all was lysed)
  • In the final elution step, used only 50 uL rather than 200 uL, as Shanelle mentioned that she had success with previous Tanner crab samples following that method
  • Samples are currently stored in -20 (plate labeled with my name and date, samples labeled 1, 2, and 4, as 3 was discarded)

Important notes on use of Qubit:

  • Ran both standards first
  • Used 2 uL of sample (post-extraction of course), 198 uL of buffer

Alright, final concentrations as determined by Qubit:

Sample 1: 14.9 ng/ul

Sample 2: 2.18 ng/ul

Sample 3: failed to pellet

Sample 4: 15.3 ng/ul